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Invitrogen
CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), Super Bright™ 600, eBioscience™
FIGURE: 1 / 16
CD90.1 (Thy-1.1) Antibody (63-0900-80) in Flow
Staining of rat splenocytes with CD3 Monoclonal Antibody, APC (Product # 17-0030-82) and 0.125 µg of Mouse IgG2a kappa Isotype Control Super Bright 600 (Product # 63-4724-82) (left) or 0.125 µg of CD90-1 (Thy-1-1) Monoclonal Antibody, Super Bright 600 (right). Total viable cells were used for analysis.
Product Details
63-0900-80
Applications
Tested Dilution
Publications
Product Specifications
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HIS51
Conjugate
Excitation/Emission Max
414/601 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_2717050
Product Specific Information
Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.
Applications Reported: This HIS51 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HIS51 antibody has been tested by flow cytometric analysis of rat splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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